RIPK1 Knockout cell line(HT1080)

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location： Home > Products > KO Cell Lines > RIPK1 Knockout cell line(HT1080)

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RIPK1 Knockout cell line(HT1080) Order

RIPK1 Knockout cell line(HT1080)
Catalog#
YKO-HJ060
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RIPK1 Knockout cell line(HT1080)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RIPK1 Knockout cell line (HT1080)
Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:4
Species
Human
Gene Name
RIPK1
Gene ID
8737
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 2 (based on transcript RIPK1-201)
Validation: PCR+Sanger Sequencing
RIPK1 Knockout cell line(HT1080) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HT-1080		 STR Info (Cell bank)
Cell Line:HT-1080
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11131113
D13S317 12141214
D7S820 910910
D16S539 912912
VWA 14191419
TH01 6666
AMEL XYXY
TPOX 8888
CSF1PO 12121212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RIPK1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HT-1080 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RIPK1 gene knockout RIPK1 Knockout cell line (HT1080) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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